4. Gold chip

Feature of Gold chip


Bare gold chip

A 17- mm2 glass plate in which one side is covered with very thin gold particle, by which the incident and reflection light gives a real time response is called “Gold chip for handy SPR”. This gold surface is covered with a transparent sheet containing suitable flow channel. The transparent sheet has three layers- the middle is elastic type and the other sides are covered with transparent layer.








Batch cell(Silicon):

Batch cell

How to Use
1. A small drop of matching oil put onto prism
2. Place a gold chip onto prism and carefully remove bubbles between chip and prism.
3. Put a silicon batch cell on the gold chip
4. Using a drop of water or buffer onto gold surface, a typical SPR curve should be observed when light passes over the prism.

How to set up batch cell


A typical SPR curve using batch cell in water



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Gold chip surface
Gold chip with transparent sheetGold chip
Gold chip with PDMS

Application of Gold Chip on SPR system:

We can observe the changes of angles in two different ways using handy SPR evaluation kit. (1) Batch (drop) cell, and (2) Flow cell.



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Flow Cell System

Flow cell Flow cell

How to Use

1. A small drop of matching oil is put onto prism
2. PDMS flow cell is put onto gold surface
3. Place the gold surface containing PDMS on the prism
4. Syringe pump is used for continuous flow system.

A typical SPR curve using batch cell in PBS


Catalog No. Description
012072 Handy SPR PS-0109
Components
Transmission cable
Power cable
Software
011870 Matching oil
011869 Gold chip
011871 PDMS flow cell
Click here to contact ALS.




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last modified 2014/02/05