3. Instrument setup

Instrument setting:

1. At the first stage, a cable is connected to Handy SPR port. Next, power supply
   adaptor is connected to Handy SPR.

Connecting a cable
Connecting a power cable

2. The other side of the cable is connected to the port of PC, and the plug of
   a power cable is connected to a wall socket.

Cnnecting a cable to a PC

3. Then the power switch of Handy-SPR is turned 'ON'. Blue LED lights up.


a Blue indication light

Program installation

A program CD includes with Handy SPR evaluation Kit that is easy to install into PC with Windows 98, 2000 and XP. Excel program is required to analyze SPR data. This program CD contains both application and analysis software.

The procedure of CD installation into PC is shown in as follows. However, depending on PC configurations, the procedure may differ from PC to PC.
1.Insert Program CD into CD driver of PC
2.Open CD on PC and click on “setup.exe” to perform the installation
3.Follow the direction of installation program
4.A folder called "SPR win3" (SPR application software) will appear on Windows menu
5.Installation is completed


In this consequence, the minimum requirements of PC's configuration for Handy SPR application that we usually recommend are as follows-

  • CPU- Pentium or Celeron with 750 MHz or more
  • HDD-20 MB or more
  • Memory-256 MB or more
  • OS-Windows XP

Operating Procedure of SPR program:

1. At the beginning, connect the PC cable between SPR instrument and PC. Simultaneously, connect power supply cable and turn on the power, a blue light appears. Then, double click on "SPR win3" software. As a result, the following windows will appear.

Application - screen image

Point to be noted: Be sure that the light of power supply is on before opening the software program. Without power supply on, software program will not open.

Program Settings:

In the window called “ Handy-SPR Control Program”, click on the settings caption at the menu bar and go through “Operate Settings” caption. After words, a new window will open. There are three setting items that will appear on the Operate setting windows like-
(i) Linear CCD Settings, (ii) SPR Peak Detect and (iii) SPR Angle Calibration.

Program settings - Operate Settings

At the same time, light source for SPR measurement is arranged via Linear CCD Settings window. The current value of LED, CCD Exposure time and the number of data should be arranged here.

Then, click on SPR Peak Detect icon on Operate Settings bar. A new window will appear that is shown in below. Now, select a suitable threshold level and angle ranges to observe a good SPR curve.

Program settings - SPR peak detect

Again click on SPR Angle Calibration and a new window will open. It is possible to set the SPR angle and pixel number on linear CCD to incident angle.

Operate settings - SPR angle calibration

Finally, click on OK to activate the Operate Settings bar.

Measurement settings:

Measurement interval should be checked before measurement process starts for data saving at a specific interval. A measurement interval can be changed at 3, 5, 15 seconds etc.


Measurement settings - Total running time window

The button is clicked continuously for starting the measurement. To terminate the measurement, 'Stop' button should be clicked.

Measurement settings - Continuous/Stop button

During the measurement, the SPR curve with angle changes (deg) against intensity is displayed on left side graph for every measurement. In a real time measurement, the changes of SPR angle and intensity with the moving time are displayed on the bottom of right- side (red circle).

Measurement settings - SPR peak info window


Catalog No. Description
012072 Handy SPR PS-0109
Components
Transmission cable
Power cable
Software
011870 Matching oil
011869 Gold chip
011871 PDMS flow cell
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last modified 2014/02/05